Phospho specific antibody production
WebThe affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing the phosphospecificity of the antibody will be described here. … WebMonoclonal Antibody for studying ERK1 (Thr202/Tyr204) phosphate/ERK2 (Thr185/Tyr187) phosphate. Cited in 950 publications. Validated for WB, IP, IHC. Highly specific and rigorously validated in-house, Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit Monoclonal Antibody (CST #4376) is ready to ship.
Phospho specific antibody production
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WebCreative Diagnostics offers extensive selection of phosphor-specific antibodies, which can be used in a variety of applications including Western blot, immunoprecipitations, … WebSep 17, 2003 · Phospho-specific antibodies were labeled with Alexa Fluor dyes to provide multicolor analysis of different signaling events simultaneously within individual cells. Results Fixing cells with 1.5% formaldehyde followed by permeabilization in methanol gave optimal results for pERK, pp38, pJNK, pStat1, pStat5, and pStat6 staining.
WebPhospho-specific antibodies are widely used to identify the activation of signal transduction pathways resulting from the phosphorylation of a diverse set of proteins that include … WebCreative Biolabs offers high-quality Rabbit Anti-NDRG1 (phospho S330) Monoclonal Antibody (PZR5594), Unconjugated to boost neuroscience research. Toggle navigation. Service . In Vitro Services. Cell Culture Models; ... Antidepressant Actions of Ketamine Engage Cell-Specific Translation via eIF4E;
WebRabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1/ABL2. Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y393/Y429 of human ABL1/ABL2. (PAB12190) - Products - Abnova WebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques. 26 Several phospho …
WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein.
WebThese proteins have been produced recombinantly and used routinely for affinity purification of key antibody types from a variety of species. Protein A/G purification is a quick purification method for polyclonal antibodies that have been generated against recombinant proteins or antigens. husky 947265 air compressor repairsWebAug 12, 2016 · Antibodies raised by peptides have become essential tools in life science research. Virtually all phospho-specific antibodies are now produced using phosphopeptides as antigens. Typically,... hush of mayfairWebJan 24, 2011 · Phospho-specific antibodies that recognize specific phospho-sites can facilitate elucidation of the signaling pathway inducing phosphorylation as well as the … husky health rx pcn numberWebThis antibody is predicted to react with mouse based on 100% sequence homology. In Western blot, this antibody is specific for the ∽82 kDa rabphilin protein phosphorylated at Ser234. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. husk coffee sheffieldWebIn fact, Phospho-Specific Antibodies developed by our expert scientists detect specific protein phosphorylation with high sensitivity even within complex cellular protein mixtures. Additional Advantages of Peptide Antigens Lower probability of cross-reactivity More flexibility in antigen selection Only protein sequence is needed husky cart with wheelsWebPhosphorylation site-specific antibodies enable analysis of key targets in normal and disease states, including cardiovascular disease, cancer, inflammation, … husky adoption massachusettsWebPhospho-specific antibodies, targeting at protein phosphorylation, are one of the recently well-developed and popularly-searched antibodies. Protein phosphorylation constitutes a key post-translational modification involved in regulation of cell signaling pathways governing a diversity of cellular processes via two mechanisms. husky blizzard 60% software